中国人兽共患病学报2013，Vol.29Issue(7)：700-703,708,5.DOI:10.3969/cjz.j.issn.1002-2694.2013.07.012
p38MAPK抑制剂SB202190体外对细粒棘球蚴原头节的作用
In vitro effects of p38MAPK inhibitor SB202190 on Echinococcus granulosus protoscoleces
摘要
Abstract
The aim of this study was to investigate the in vitro efficacy of the p38MAPK inhibitor SB202190 against Echinococcus granulosus protoscoleces.Protoscolices of Echinococcusgranulosus were incubated with SB202190 at concentrations of 12.5,25,50,100μmol/L,and the effects on protoscoleces viability were monitored by 0.1％ eosin staining and light microscopic inspection.Each experiment was repeated for three times.Starting from day 1 of incubation in the presence of 50 μmol/L and 100μmol/L SB202190,viability of protoscoleces started to decline depending on the concentration of the inhibitors.After 14 days of incubation in 100μmol/L of SB202190,no survival protoscolece remained but 13.8％ survival proto scoleces were found in 50μmol/L of SB202190 after 14 days.At the same time,ultrastructural effects of protoscoleces were observed by scanning electron microscopy (SEM),the morphological changes included contraction of the soma region,formation of blebs on the tegument,and rostellar disorganization.Additionally,caspase-3 was detected in protoscoleces and the result showed that the activity of caspase-3 was found higher in protoscoleces incubated with SB202190 as compared to the control protoscoleces.The data reported in this article demonstrate a clear in vitro effect of SB202190 against E.granulosus protoscoleces.关键词
p38MAPK抑制剂/细粒棘球蚴原头节/体外实验Key words
p38MAPK inhibitor/ Echinococcus granulosus protoscolices protoscolices/ in vitro分类
医药卫生引用本文复制引用
张晶,吕海龙,王成华,孙冯,雷颖,彭心宇,姜玉峰..p38MAPK抑制剂SB202190体外对细粒棘球蚴原头节的作用[J].中国人兽共患病学报,2013,29(7):700-703,708,5.基金项目
Supported by the National Natural Science Foundation of China (No.30960338),the Xinjiang Production and Construction Corps Foundation (Nos.2011BB019 & 2011BA058),and the Shihezi University Foundation (Nos.RCZX200925 & LHJJ2010B10) （No.30960338）
国家自然科学基金(30960338) （30960338）
兵团博士基金(2011BB019) （2011BB019）
兵团重点领域科技攻关(2011BA058) （2011BA058）
石河子大学(RCZX200925,LHJJ2010B10)联合资助 （RCZX200925,LHJJ2010B10）
