中国人兽共患病学报2013，Vol.29Issue(8)：748-752,5.DOI:10.3969/cjz.j.issn.1002-2694.2013.08.003
以棋盘法测定汉防己甲素与伊曲康唑的体外联合抗烟曲霉作用
In vitro interactions between tetrandrine and itraconazole against Aspergillus fumigatus determined by the checkerboard method
摘要
Abstract
The purpose of this paper is to detect the in vitro antifungal interactions between tetrandrine (TET) and itraconazole (ITC) against Aspergillus fumigatus.According to the Clinical and Laboratory Standards Institute (CLSI) M38 A2 guidelines,we used 21 clinical isolates of Aspergillus fumigatus to evaluate the response through the checkerboard microdilution method.We employed the spectrophotometric method to determine the results of the interactions,and the nature of interactions was assessed by the fractional inhibitory concentration index (FICI) model.The result was also verified by isobologram with AF46 as sample.The MICs of TET and ITC alone were 256-512 pg/mL and 0.125-32μg/mL,respectively.While the MICs of TET and ITC in combination were 8-64 μg/mL and 0.03-2μg/mL,with the disappearance of the so-called "trailing"phenomenon.The FICI of the drugs in combination ranged from 0.08-0.38,revealing strong synergism between TET and ITC.Synergism was also demonstrated that the corresponding isobole of AF46 was concave.Our findings suggest that when combined with ITC,TET show potent synergism in antifungal activity.关键词
汉防己甲素/伊曲康唑/烟曲霉/增效作用/棋盘法Key words
tetrandrine/itraconazole/Aspergillus fumigatus/synergistic effect/checkerboard microdilution method分类
医药卫生引用本文复制引用
吴锐,刁文琦,宋延君,李水秀,吕霞琳,王露霞,刘维达,张宏..以棋盘法测定汉防己甲素与伊曲康唑的体外联合抗烟曲霉作用[J].中国人兽共患病学报,2013,29(8):748-752,5.基金项目
Supported by grants from the National Natural Science Foundation of China (No.81171542),the Science and Technology Planning Project of Guangdong Province,China (No.2009B030801015),the Guangzhou Key Technology R&D Program,China (No.2010J-E011),and the Fundamental Research Funds for the Central Universities (No.21611509) （No.81171542）
国家自然科学基金(No.81171542),广东省科技计划项目(No.2009B030801015),广州市科技支撑项目(No.2010J-E011)和中央高校基本科研业务费专项资金(No.21611509)联合资助 （No.81171542）
