中国水稻科学2013，Vol.27Issue(4)：359-364,6.DOI:10.3969/j.issn.1001-7216.2013.04.004
水稻幼苗叶色温敏感突变体的鉴定及其基因定位
Identification and Gene Mapping of a Thermo-sensitive Leaf-color Mutant at Seedling Stage in Rice
摘要
Abstract
A thermo-sensitive leaf-color mutant,tsl1 1,was selected from the progeny of the japonica rice variety Jiahua 1 by 60Co γ-radiation.Compared with the wild-type parent Jiahua 1 at 20℃C,the mutant was characterized yellowseedling,decreased contents of chlorophyll a,chlorophyll b and carotenoid by 94.6 ％,88.2 ％ and 91.5 ％,respectively.Simultaneously,the result of electron microscopic observation revealed that the structure of chloroplasts in the tsl11 was imperfect seriously.However,the mutant was similar to wild type at high temperatures (32℃C).These results indicated that the mutant was obviously thermo-sensitive.Genetic analysis showed that the mutant trait was controlledby a single nuclear recessive gene,tentatively designated as tsl11.Moreover,the mapping population of 4928 F2 mutant individuals derived from Pei'ai 64S/tsl11 were constructed,then using SSR and InDel markers,the tsl11 gene was finally mapped to a region of 147 kb between ID14506 and ID14653 on chromosome 11.关键词
温敏感/叶色突变体/叶绿素含量/基因定位Key words
thermo-sensitive/ leaf-color mutant/ chlorophyll content/ gene mapping分类
生物科学引用本文复制引用
江少华,周华,林冬枝,董彦君,叶胜海,张小明..水稻幼苗叶色温敏感突变体的鉴定及其基因定位[J].中国水稻科学,2013,27(4):359-364,6.基金项目
国家自然科学基金资助项目(30971552) （30971552）
上海市科委项目(09DJ1400505,0939191200,10DZ2271800,12ZR1422000) （09DJ1400505,0939191200,10DZ2271800,12ZR1422000）
上海市教委项目(J50401). （J50401）
