中国兽医科学2013，Vol.43Issue(8)：822-827,6.
PRRSV体外感染Marc-145细胞对NF-κB p65/p50转录时相及核易位的影响
Influence of PRRSV infection to Marc-145 cells in vitro on NF-κB p65/p50 transcription phase and nuclear translocation
摘要
Abstract
To study the influence of PRRSV infection on mRNA transcription phase and nuclear translocation of nuclear factor-κB(NF-κB,p65/p50) in vitro,and to investigate whether PRRSV induces immunologic suppression related to NF-κB abnormal activation,the p65/p50 genes mRNA transcription levels of Marc-145 cells infected with PRRSV in different periods were analyzed using real-time fluorescence quantitative PCR(real-time FQ-PCR).Nuclear proteins of Marc-145 cells infected with PRRSV in different periods were extracted,and the activity levels of p65/p50 in the cellular nuclear were detected by Western-blot.Real-time FQ-PCR results indicated that,the p65/p50 mRNA transcription levels of Marc-145 cells decreased rapidly at 8-12 hour post-infection(hpi),then kept at a low level at 12-120 hpi,which was significantly lower than the levels of control group at the same time and Marc-145 cells without PRRSV infection (P＜0.05 or P＜0.01).The p50 expression in the celluar nuclear could not be detected at 12-96 hpi by Western-blot,neither the p65 at 12 hpi.The results revealed that PRRSV infection in vitro would inhibit NF-κB p65/p50 mRNA transcription and nuclear translocation.The PRRSV-induced immunologic suppression may be related to the low activation level of NF-κB.This study would provide some new ideas for further studies on the pathogenic mechanism of PRRSV and the function of NF-κB.关键词
猪繁殖与呼吸综合征病毒/细胞核转录因子-κappaB/转录时相/核易位Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/nuclear factor-κB(NF-κB) /transcription phase/ nuclear translocation分类
农业科技引用本文复制引用
王潇娣,朱玲,周远成,陈蕾,李碧,蔡雨函,徐志文..PRRSV体外感染Marc-145细胞对NF-κB p65/p50转录时相及核易位的影响[J].中国兽医科学,2013,43(8):822-827,6.基金项目
四川省科技支撑计划项目(2011FZ0065,2012NZ0001) （2011FZ0065,2012NZ0001）
