中国兽医科学2013，Vol.43Issue(8)：866-870,5.
旋毛虫感染小鼠后TNF-α诱导肺细胞凋亡的观察
Experimental observation of TNF-α-induced pulmonary apoptosis in the mice infected with Trichinella spiralis
摘要
Abstract
To explore the pathogenic role and signal transduction pathways of TNF-α-induced lung injury of the mice infected with Trichinella spiralis,the mRNA expression levels of TNF-α gene,the apoptosis genes and the anti-apoptosis genes both in the lungs without injury and in the injured lungs were monitored using semi-quantitative RT-PCR.The morphologic features of the cells undergoing apoptosis were observed using TUNEL at different time points during Trichinella spiralis infection.In result,the relative expression level of TNF-α was from 0.74 (before infection)to 1.19(on day 4 post-infection),and the lung cells of apoptosis increased.On day 14 post-infection,it reached peak(1.33),then decreased to 1.11 on day 35 post-infection.Apoptosis index,meanwhile,increased from 2.57 ％ to 21.42 ％ (P＜0.05),and then decreased to 14.74％.The relative level of TNFR1,FADD,Caspase8,Caspase3,TRAF2,RIP and NF-κB showed a similar tendency to TNF-α' s.TNF-α induced severity of lung cell apoptosis,which is related to the different stages of Trichinella life.关键词
旋毛虫/TNF-α/细胞凋亡/肺损伤Key words
Trichinella spiralis / TNF-α / apoptosis / lung injury分类
农业科技引用本文复制引用
李兴超,李巍,徐佳,刘畅,俞昭阳,李晓云,宋铭忻,张唯哲..旋毛虫感染小鼠后TNF-α诱导肺细胞凋亡的观察[J].中国兽医科学,2013,43(8):866-870,5.基金项目
"十一五"国家科技支撑计划重大项目(2010BAD04B01) （2010BAD04B01）
国家自然科学基金项目(31172312) （31172312）
