中国药房2013，Vol.24Issue(31)：2949-2950,2.DOI:10.6039/j.issn.1001-0408.2013.31.25
HPLC法测定益肾蠲痹丸(浓缩)中淫羊藿苷的含量
Content Determination of Icariin in Yishen Juanbi (Concentrated) Pills by HPLC
庄旭琴 1朱文燕1
作者信息
- 1. 南京医科大学附属淮安第一医院,江苏淮安223300
- 折叠
摘要
Abstract
To establish a method for the content determination of icariin in Yishen juanbi pills.METHODS:HPLC method was adopted.The determination was performed on Kromasil C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-water (30 ∶ 70,V/V) at the flow rate of 1.0 ml/min.The column temperature was 35 ℃,and the detection wavelength was set at 270 nm.RESULTS:The linear range of icariin were 0.081 3-1.219 2 μg(r=0.999 5) with an average recovery of 100.66％ (RSD=1.92％,n=9).The RSDs of precision,reproducibility and stability tests were lower than 2％.CONCLUSIONS:The method is simple,accurate and reproducible,and it can be used for the quality control of Yishen juanbi pills.关键词
益肾蠲痹丸/淫羊藿苷/高效液相色谱法/含量测定Key words
Yishen juanbi pills/Icariin/HPLC/Content determination分类
医药卫生引用本文复制引用
庄旭琴,朱文燕..HPLC法测定益肾蠲痹丸(浓缩)中淫羊藿苷的含量[J].中国药房,2013,24(31):2949-2950,2.
