中国医科大学学报2013，Vol.42Issue(8)：710-713,4.
sCD40L诱导人脐静脉内皮细胞损伤对人脐间充质干细胞趋化性的影响
Chemotaxis of Human Umbilical Cord Mesenchymal Stem Cells to the sCD40L-induced Vascular Endothelial Cells
摘要
Abstract
Objective To study the chemotaxis effect of human umbilical cord mesenchymal stem cells to r-CD40L HUVGC.Methods The cultured HUVEC were treated by different concentration of r-CD40L factor.24 hours later,endothelial permeability was measured by fluorescein isothiocyanate labeled bovineserum albumin (FITC.BSA) clearance across the monolayer.The cultured HUVEC were divided into four groups and induced by different concentration of r-CD40L factor for 24hs.The culture solution of the four groups was collected in a sterile EP,centrifuged the solution,then added into the lower chamber of Transwell; then,the fifth passage of UC-MSCs (1 × 105/mL) was filled to the upper chamber of Transwell.After 24 h of incubation in a 5％ CO2 atmosphere at 37 ℃C,the invasive cells of the lower surface were fixed and stained by 1％ crystal violet solution.Then the cells were redyeing with tetrabromofluorescein.The extent of invasion was determined under a light microscope by counting the number of stained cells on the membranes in six randomly selected,non-overlapping fields at 200× magnification.Results r-CD40L induction lead to a significant increase in the permeabihty.The number of stained UC-MSCs on the membranes of different groups were:10.00 ± 1.76,25.96 ±2.84,37.88 ±3.23,49.04 ±2.81.The differences were statistically significant,P ＜0.01.Conclusion sCD40L could induce a significant increase in the endothehal permeability,and the r-CD40L-induced HUVEC cell injury could increase the chemotactic characteristic of UC-MSCs.关键词
子痫前期/趋化性/可溶性细胞分化抗原40L/人脐静脉内皮细胞/脐带间充质干细胞Key words
preeclampsia/ chemotaxis/ sCD40L/ human umbilical veins endothelial cell/ human umbilical cord mesenchymal stem cells分类
医药卫生引用本文复制引用
史丽,赵喜娃,尹洁,李青梅,董晓静..sCD40L诱导人脐静脉内皮细胞损伤对人脐间充质干细胞趋化性的影响[J].中国医科大学学报,2013,42(8):710-713,4.基金项目
河北省医学科学研究重点课题(20120122) （20120122）
