中国医科大学学报2013，Vol.42Issue(8)：726-729,4.
GPC3基因重组慢病毒载体的构建及其在肝癌细胞中的表达
Construction of shRNA Lentiviral Vector Targeting Human GPC3 Gene and Its Expression in HepG2 Cells
摘要
Abstract
Objective To construct shRNA lentiviral vectors targeting human GPC3 gene and observe the transfection and expression level in HepG2 cells.Methods The siRNA sequences specifically targeting the GPC3 gene were designed and cloned into lentiviral vector pGLV3-GFP using DNA recombinant technique.The 293T cells were transfected by lipofectin reagent for lentiviral particles packaged,and viral titer was determined.GPC3 mRNA and protein expression were examined by RT-PCR and Western blot.Restlts The recombinant lentiviral vectors were successfully constructed and the virus reached a titer of 1×108 TU/mL.The expression of GPC3 was significantly decreased after infection with the lentivirus.Conclusion shRNA expressing lentiviral recombinants targeting the GPC3 gene were successfully constructed,which provides a basis for the further functional study of GPC3.关键词
GPC3/RNA干扰/慢病毒载体Key words
GPC3/ RNAi/ lentiviral vector分类
医药卫生引用本文复制引用
祁馨卉,崔慧霞,马楠,姜又红..GPC3基因重组慢病毒载体的构建及其在肝癌细胞中的表达[J].中国医科大学学报,2013,42(8):726-729,4.基金项目
辽宁省科学技术计划项目(2011415052-3) （2011415052-3）
