中国药物经济学Issue(5)：80-83,4.
U251胶质瘤细胞系中脑肿瘤干细胞的分离、培养及鉴定
Isolation,Culture and Identification of Brain Tumor Stem Cellswithin U251 Glioma Cell Line In Vitro
陈成 1芦明 2李茗初 3方加胜4
作者信息
- 1. 深圳市人民医院神经外科,广东深圳 518020
- 2. 杭州市萧山区第一人民医院神经外科,浙江杭州 311200
- 3. 北京市宣武医院神经外科,北京 100053
- 4. 中南大学湘雅医院神经外科,湖南长沙 410008
- 折叠
摘要
Abstract
Objective This study was to isolate, culture and identificate brain tumor stem cel s from U251 Gliome cel line in vitro and observe their growth pattern.To establish a fundation for further reseach of brain tumor stem cel . Methods U251cel s were seeded in serum-free DMEM-F12 medium supplemented with B27, EGF and bFGF in 6-wel plates, after U251 brain tumor spheres formed, they were dissociated and passaged in fresh medium periodical y. U251 brain tumor spheres and the single cel s of them were induced to differentiate in the medium with 10%FBS supplemented. U251cel s cultivate in serum-supplemented medium and serum-free medium alternately. At last we performed immunocytochemistry of U251 brain tumor spheres for CD133 and Nestin. Results Some U251 glioma cel have the capacity to self-renew, proliferate and generate free-floating neurosphere-like brain tumor spheres in serum-free medium in vitro. U251 glioma spheres and the single cel s of them can be induced to differentiate and adherent to the bottom of the culture plates. The growing patterns of U251 glioma cel line can be converted by changing the culture mediums. The brain tumor stem cel are CD133+Nestin+cel s. Conclusion The U251 glioma cel line contain brain tumor stem cel s which can be isolated, proliferated and differentiated in vitro. The research of brain tumor stem cel s may provide a new platform for brain tumor study.关键词
U251胶质瘤细胞系/脑肿瘤干细胞/分离培养/分化Key words
U251 glioma cel line/Brain tumor stem cel/Isolation/Cel differentiation引用本文复制引用
陈成,芦明,李茗初,方加胜..U251胶质瘤细胞系中脑肿瘤干细胞的分离、培养及鉴定[J].中国药物经济学,2012,(5):80-83,4.
