中国医学装备2013，Vol.10Issue(8)：1-5,5.DOI:10.3969/J.ISSN.1672-8270.2013.08.001
基于罗叶泵的搏动式组织工程培养体系的构建
Design and construction of Luo-Ye pump-based pulsatile tissue engineering culture system
摘要
Abstract
Objective:To design and set up Luo-Ye pump-based pulsatile tissue engineering culture system and complete the basic performance test.Methods:According to the needs of vascular tissue engineering,the bioreactor was designed.The main part of bioreactor is made of glass.Starting and adjusting the output pressure and frequency of Luo-Ye pump for monitoring fluid pressure and frequency by ECG and measure the actual flow by flow meter.Results:The bioreactor is mainly made of glass.The pulsatile system ran no leakage or loosely after 14days running.The pressure waveform in ECG is pulsatile.If the output trequency is tixed,the pressure and tlow in bioreactor have been measured and the flow in bioreactor increased with the increase of pump output pressure.If the output pressure is fixed,the pressure and flow in bioreactor have been measured and the flow in bioreactor increased with the increase of pump output pressure too.Conclusion:LuoYe pump-based pulsatile tissue engineering culture system has good stability.It can produce pulsating stimulation and apply in vascular tissue engineering three-dimensional culture.The pulsatile system has different fluids by adjusting the output pressure,frequency and other parameters of Luo Ye pump,and can be used to adjust the microenvironment for cardiovascular tissue engineering.关键词
组织工程/培养体系/罗叶泵/搏动/生物反应器Key words
Tissue engineering/ Culture system/ Luo Ye pump/ Pulsatile/ Bioreactor分类
医药卫生引用本文复制引用
吴岳恒,黄焕雷,周嘉辉,成安衡,余细勇,林展翼,庄建,肖学钧..基于罗叶泵的搏动式组织工程培养体系的构建[J].中国医学装备,2013,10(8):1-5,5.基金项目
国家自然科学基金(30901468)"偏磷酸钙改善多聚四羟基丁酸膜细胞亲和性的研究" （30901468）
广东省教育部产学研结合项目基金(2008B090500210)"气动心室辅助装置的研究及临床应用" （2008B090500210）
广东省科技计划项目(2009B060700116)"近似人体搏动压力环境的平滑肌细胞三维培养方法研究" （2009B060700116）
广东省自然科学资金(1015100800800011)"脉动式张应力对人脐动脉VSMC增殖分化的诱导作用" （1015100800800011）
