中国药业2013，Vol.22Issue(15)：54-56,3.
高效液相色谱法同时测定人体润滑剂中苯氧乙醇和羟苯甲酯含量
HPLC Method for Simultaneous Determination of Contents of Phenoxyethanol and Methyl Hydroxybenzoate in Human Body Lubricant
张成 1王刚林 2崔平1
作者信息
- 1. 陕西省医疗器械检测中心,陕西西安 710075
- 2. 重庆医疗器械质量检验中心,重庆401147
- 折叠
摘要
Abstract
Objective To establish a HPLC mothod of simultaneous determination of the phenoxyethanol and methyl hydroxybenzoate contents in the human body lubricant.Methods The Acclaim 120 C18 column (150 mm ×4.6 mm,5 μm) was adopted with the gradient elution,acetonitrile-water-glacial acetic acid (310∶690∶2) as the mobile phase A and acetonitrile as the mobile phase B.The detection wavelength was 265 nm.Results Phenoxyethanol and methyl hydroxybenzoate were good linear in the range of 3.02-60.39 μg/mL and 1.33-26.64 μg/mL,the linear equation was Y=0.086X+0.004 34(r=0.999 99,n=5) and Y=0.598 8X+0.017 39 (r =0.999 99,n =5) respectively,the average recovery rates were 95.66％ (RSD =0.92％) and 96.37％ (RSD =0.53％) respectively.The contents of phenoxyethanol and methyl hydroxybenzoate were 2.85 mg/g and 2.00 mg/g respectively in the human body lubricant.Conclusion This method is simple,reliable and can be used for the determination of phenoxyethanol and methyl hydroxybenzoate in the human body lubricant.关键词
高效液相色谱法/苯氧乙醇/羟苯甲酯/人体润滑剂Key words
HPLC/phenoxyethanol/methyl hydroxybenzoate/human body lubricant分类
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张成,王刚林,崔平..高效液相色谱法同时测定人体润滑剂中苯氧乙醇和羟苯甲酯含量[J].中国药业,2013,22(15):54-56,3.
