中国医药科学Issue(16)：103-104,110,3.
荧光原位杂交检测宫颈细胞hTERC基因扩增及其临床意义
Clinical significance of hTERC gene amplification in cervical squamous epithelial cells detected by fluorescence in situ hybridization
钟守军 1刘晓萍 1米贤军 1段立锋 1杨伟洪 1陈志强1
作者信息
- 1. 广东省中山市博爱医院病理科,广东中山528403
- 折叠
摘要
Abstract
Objective To study the relationship and clinical significance between human telomerase gene amplification and development of cervical intraepithelial neoplasm and cervical squamous cancer. Methods hTERC gene amplification were detected by FISH in cytological samples from 100 cases of CIN /cervical squamous cancer and 20 cases of normal cervix. Results In 100 cases of various types of cervical cell lesions, the abnormal amplification ratio of hTERC gene was:13.3%(4/30) in CIN1 group,71.0%(22/31) in CIN2 group, CIN 3 group and cervical cancer squamous group were 100%(19/19,20/20), each group compare difference to all have statistical significance(P <0.01). Conclusion hTERC gene was amplified both in CIN and cervical squamous cancer cells, and the amplification rate increases with lesion progression. The amplification rate of high grade CIN and squamous cancer was significantly higher than that of low grade CIN. Detection of hTERC gene amplification can be used as an important indicator in cervical CIN and cervical cancer screening and prognostic evaluation.关键词
宫颈上皮内肿瘤/宫颈癌/hTERC基因/荧光原位杂交技术Key words
Cervical intraepithelial neoplasm/Cervical cancer/hTERC gene/Fluorescence in situ hybridization分类
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钟守军,刘晓萍,米贤军,段立锋,杨伟洪,陈志强..荧光原位杂交检测宫颈细胞hTERC基因扩增及其临床意义[J].中国医药科学,2012,(16):103-104,110,3.
