中国肿瘤生物治疗杂志2013，Vol.20Issue(4)：414-418,5.DOI:10.3872/j.issn.1007-385X.2013.04.006
慢病毒介导NDRG2基因过表达抑制人膀胱癌T24细胞的侵袭和迁移
Lentivirus-mediated NDRG2 overexpression inhibits invasion and metastasis of human bladder cancer T24 cells
摘要
Abstract
Objective:To observe the effect of NDRG2 (N-myc downstream-regulated gene 2) overexpression on invasion and metastasis of human bladder cancer T24 cell in vitro.Methods:The T24 cells were infected with lentivirus carrying NDRG2 gene (Lenti-NDRG2) to construct a stable cell line overexpression NDRG2.Western blotting was performed to examine the expression of NDRG2,matrix metalloproteinase (MMP)-2 and MMP-9 in T24 cells.The effects of NDRG2 overexpression on the invasion and migration of T24 cells were examined by the transwell invasion and migration assays,respectively.Results:The T24 cell line stable overexpression NDRG2 was successfully constructed.Compared with the LEN-LacZ group and the control group,the NDRG2 expression was efficiently increased in the Lenti-NDRG2 group ([30.1±1.27] vs [9.9±1.24],[8.2 ±1.28]; P＜0.01); the MMP-2 ([13.5 ±1.32] vs [30.7 ±1.29],[28.8 ± 1.30] ; P ＜0.01) and MMP-9 expressions ([11.7 ±1.27] vs [25.2 ± 1.28],[26.4 ± 1.31] ; P ＜0.01) in T24 cells of Lenti-NDRG2 group were significantly suppressed; the capabilities of the invasion ([18.1 ± 3.4] vs [88.5 ±4.2],[90.2 ±4.1] ; P＜0.01)and migration ([20.1 ±3.5] vs [109.4±5.6],[113.0 ±4.9] ; P ＜0.01) of T24cells were significantly suppressed in the Lenti-NDRG2 group.Conclusion:The lentivirus-mediated NDRG2 overexpression can suppress the invasion and migration of human bladder cancer T24 cells.关键词
膀胱癌/T24细胞/N-Myc下游调节基因-2/侵袭/迁移Key words
bladder cancer/ T24 cell / N-Myc downstream-regulated gene 2 (NDRG2) / invasion/ metastasis分类
医药卫生引用本文复制引用
李瑞晓,于垂恭,张璟,王禾,武国军..慢病毒介导NDRG2基因过表达抑制人膀胱癌T24细胞的侵袭和迁移[J].中国肿瘤生物治疗杂志,2013,20(4):414-418,5.基金项目
国家自然科学基金资助项目(No.31070681).Project supported by the National Natural Science Foundation of China(No.31070681) （No.31070681）
