肿瘤防治研究2013，Vol.40Issue(8)：758-761,4.DOI:10.3971/j.issn.1000-8578.2013.08.008
雷帕霉素对骨肉瘤细胞MG63核干细胞因子表达的调节
Effect of Rapamycin on Nucleostemin Expression in Osteosarcoma MG63 Cells
刘郁东 1郑启新 1吴宏斌 1郭晓东 1李景峰 1郝少飞1
作者信息
- 1. 430023武汉,华中科技大学同济医学院附属协和医院骨科
- 折叠
摘要
Abstract
Objective To investigate the regulation and possible mechanisms of rapamycin (RAPA) on nucleostemin (NS) expression in osteosarcoma MG63 cells.Methods After treatment with RAPA or in combination with various kinase inhibitors pre-treatment,the mRNA and protein expression of NS were determined by reverse transcription polymerase chine reaction (RT-PCR) and Western blot respectively.Effect of RAPA on the selected kinase was analysed by Western blot.Results RAPA up-regulated NS mRNA and protein expression and the protein kinase B (Akt) inhibitor prevented the effects of RAPA.RAPA did not affect the total Akt activity.However,the nuclear translocation of phosphorylated Akt at Ser473 (pAkt) was enhanced by RAPA.Conclusion RAPA possibly increases the expression of NS in MG63 cells through enhancing the nuclear translocation of pAkt.关键词
骨肉瘤/雷帕霉素/核干细胞因子/蛋白激酶BKey words
Osteosarcoma/Rapamycin/Nucleostemin/Protein kinase B分类
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刘郁东,郑启新,吴宏斌,郭晓东,李景峰,郝少飞..雷帕霉素对骨肉瘤细胞MG63核干细胞因子表达的调节[J].肿瘤防治研究,2013,40(8):758-761,4.
