肿瘤药学Issue(5)：322-331,10.DOI:10.3969/j.issn.2095-1264.2012.05.001
流式细胞仪在B细胞肿瘤诊断和治疗中的作用
The Role of Flow Cytometry in the Diagnosis and Treatment of B Cell Lymphoma
张丽凤1
作者信息
- 1. 宾夕法尼亚大学流式细胞术与细胞分选资源实验室,费城 宾州,19104-6082,美国
- 折叠
摘要
Abstract
Flow cytometric immunophenotyping (FCI) has become an indispensable approach for the diagnosis, classifica-tion, staging, and monitoring of B cell lymphoma. In conjunction with many other new techniques, this technique can be used to detect neoplastic cells with high sensitivity and specificity and it can be done within several hours. By using this technique, B-lymphocytes can be recognized by expression of lineage-associated antigens, the stages of maturation and belonging to dif-ferent biologic compartments;neoplastic B-cells can be distinguished from reactive B-cells by demonstration of immunoglobu-lin light chain restriction or phenotypic aberrancy;and the phenotype of the neoplastic cells can be used to identify one of the distinct disease entities. In addition, visualization of minimal residual disease, lymphoma specific antigen expression and intracel-lular signaling have played an increasing role in accessing treatment response, relapse and optimization of therapeutic strategies.关键词
流式细胞仪/B淋巴细胞瘤/诊断Key words
Flow cytometric/B cell lymphoma/Diagnosis分类
医药卫生引用本文复制引用
张丽凤..流式细胞仪在B细胞肿瘤诊断和治疗中的作用[J].肿瘤药学,2012,(5):322-331,10.
