肿瘤药学Issue(5)：343-346,350,5.DOI:10.3969/j.issn.2095-1264.2012.05.004
维生素E对顺铂所致的小鼠肾毒性的影响及其机制研究
The Effect of Vitamin E on Cisplatin Induced Nephrotoxicity in Mice and its Mechanism
张林 1刘伏友 2彭佑铭 2李大伟 1曾妮 1武蓉 1刘沧桑1
作者信息
- 1. 长沙市四医院肾内科,湖南 长沙,410006
- 2. 中南大学湘雅二医院肾脏病研究所,湖南 长沙,410008
- 折叠
摘要
Abstract
Objective To investigate the effects of vitamin E on cisplatin induced nephrotoxicity in mice and its mechanism. Methods 10 mg·kg-1 cisplatin was used to induce nephrotoxicity in mice by intraperitoneal injection, and 48 h later, 250 mg·kg-1 and 500 mg·kg-1 vitamin E were respectively used. Then at 72 h after cisplatin treatment, the serum level of urea nitrogen and creatinine were detected to evaluate the change of renal function, and the activities of malonaldehyde (MDA), superoxide dis-mutase (SOD), catalase (CAT), reduced glutathione (GSH) and glutathion peroxidase (GPX) in renal tissue were detected to evaluate the change of oxidative stress. Results Compared with normal mice, the serum level of urea nitrogen and creatinine as well as MDA in renal tissue significantly increased in 10 mg·kg-1 cisplatin-induced mice (P<0.01), while the activity of SOD, CAT, GSH and GPX in renal tissue were significantly reduced (P<0.01). Compared with 10 mg·kg-1 cisplatin-induced mice, serum level of urea nitrogen and creatinine as well as MDA in renal tissue of 250 mg·kg-1 and 500 mg·kg-1 vitamin E induced mice significantly reduced (P<0.01), and the activity of GPX in renal tissue of 250 mg·kg-1 vitamin E induced mice significantly increased (P<0.01), and so did the activity of GPX and CAT in renal tissue of 500 mg·kg-1 vitamin E induced mice (P<0.01), but no significant difference of the activity of SOD in renal tissue between the three groups was found (P>0.05). Conclusions High dose of vitamin E was effective to improve the renal function of cisplatin induced mice, and the decrease of oxidative stress may be involved.关键词
维生素E/氧化应激/肾毒性/顺铂Key words
Vitamin E/Antioxidant/Nephrotoxicity分类
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张林,刘伏友,彭佑铭,李大伟,曾妮,武蓉,刘沧桑..维生素E对顺铂所致的小鼠肾毒性的影响及其机制研究[J].肿瘤药学,2012,(5):343-346,350,5.
