肿瘤药学Issue(5)：351-355,394,6.DOI:10.3969/j.issn.2095-1264.2012.05.006
三七总皂苷对人胃癌细胞株MKN-28增殖和凋亡的影响
Effects of TSPN on the Proliferation and Apoptosis of Gastric Cancer Cell Line MKN-28
摘要
Abstract
Objective To observe the effects of total Saponins of Panax notoginseng (TSPN) on proliferation and apoptosis of human gastric cancer MKN-28 cell, and to explore whether the mechanism of apoptosis involves the up-regulation of expres-sion of death receptors-5 (DR5). Method Human gastric cancer MKN-28 cells were cultured in vitro, and their fixed-dependent growth ability were assayed by agar colony formation. The cell cycle and apoptosis rate were detected by PI staining and flow cy-tometry (FCM). The morphology of apoptotic cells was observed by Hoechst 33258 fluorescence staining under fluorescence mi-croscopy. The effect of drugs on the DR5 protein expression was analyzed by Western Bloting. Result Agar colony formation as-say showed that MKN-28 cell growth was inhibited by TSPN in a dose-dependent manner. PI staining FCM results showed that the TSPN induced apoptosis of MKN-28 cells in a time and concentration-dependent manner and the cell cycle was retarded at G1 phase. Nuclear chromatin condensation can be observed by fluorescence microscopy after Hoechst 33258 staining, and apop-totic cells were dense stained. Compared with control group, the proportion of apoptotic cells increased after TSPN treatment in a time and concentration-dependent manner. Western Bloting analysis showed that apoptosis induced by TSPN involved the up-regulation of death receptor-5 expression activity. Conclusion TSPN have a role in inhibiting proliferation and inducing apoptosis on human gastric cancer MKN-28 cells in vitro. It is inferred that this induce of apoptosis may be concerned with its role in up-regulating the expression of the death receptor-5.关键词
三七总皂苷/胃癌/凋亡/流式细胞术Key words
TSPN/Carcinoma of stomach/Apoptosis/Cytometry分类
医药卫生引用本文复制引用
吴再起,彭耀金,李有秋..三七总皂苷对人胃癌细胞株MKN-28增殖和凋亡的影响[J].肿瘤药学,2012,(5):351-355,394,6.基金项目
湖南师范大学青年科学基金项目资助(编号:81101)与湖南省教育厅基金项目资助(09C632) （编号:81101）
