肿瘤药学Issue(6)：417-421,5.DOI:10.3969/j.issn.2095-1264.2012.06.004
3-溴丙酮酸对人结肠癌 SWIII-C 细胞增值和凋亡的影响
Effects of 3-bromine pyruvate on Proliferation and Apoptosis of SWIII-C Cell
摘要
Abstract
Objective To investigate the effects of 3-bromine pyruvate on the proliferation and apoptosis of SWIII-C cells. Methods The methods with transmission electron microscope, MTT, Flow Cytometry, immunohistochemistry and so on were used to detect the changes of cellular morphology and ultramicrostructure, changes of cell proliferation, cycle distribution and apopto-sis rate, and changes of Bcl-2, c-myc and mtp53 protein expression of SWIII-C cells after giving 3-bromine pyruvate. Results 3-bromine pyruvate has the obvious inhibition effect on SWIII-C cell. Especially when its concentration reached up to 25 μg·mL-1, it achieved the best inhibition effect. The protein expression of Bcl-2, c-myc and mtp53 gene of SWIII-C cells might be regulated down by 3-bromine pyruvate. Conclusion 3-bromine pyruvate may inhibit the proliferation and induce the apoptosis of SWIII-C cells, and the mechanism may associate with the down-regulation of expression of Bcl-2, c-myc and mtp53.关键词
3- 溴丙酮酸/细胞增殖/SWIII-C细胞/细胞凋亡Key words
3-bromine pyruvate/Cell proliferation, SWIII-C cells/Cell apoptosis分类
医药卫生引用本文复制引用
刘晓红,王绍志,刘晓梅,何福海,郭丛娟,侯林中,郑学仿..3-溴丙酮酸对人结肠癌 SWIII-C 细胞增值和凋亡的影响[J].肿瘤药学,2012,(6):417-421,5.基金项目
辽宁省高校创新团队项目资助(2006T002,2009T003) （2006T002,2009T003）
