中山大学学报（自然科学版）2013，Vol.52Issue(4)：100-104,5.
AQC柱前衍生化高效液相色谱法测定宝乐果中的游离氨基酸
Application AQC as a Pre-column Derivatization Reagent for HPLC Determination of Free Amino Acids in Borojo sorbilis Cuter.
摘要
Abstract
To determine and analyze free amino acids in Borojo.The sample was derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) and then was analyzed with high-performance liquid chromatography (HPLC).HPLC was performed on a Phenomenex C18 (250mm × 4.6 mm,5 μm) column with gradient elution of deionized water (A),acetonitrile (B) and 10 mmol/L ammonium acetate buffer solution (adjusted to pH 5.10 with glacial acetic acid) (C) at the flow rate of 0.8 ～1.0 mL/min,detected with Ultra Voilet (UV) at 248 nm.Linear relationship equations were obtained with 18kinds of amino acids in the range of 0.02 ～ 0.5 mmoL/L,and their correlation coefficients were in the range of 0.998 3 ～ 0.999 2.Fourteen amino acids were detected and average recoveries of those added were in the range of 96.9 ％ ～ 106.9 ％ and RSD values were in the range of 0.67 ％ ～ 3.07 ％.The method could be used for determination of content of free amino acids in Borojo.关键词
宝乐果/AQC/游离氨基酸/柱前衍生法Key words
Borojo/ AQC/ free amino acids/ pre-column derivatization分类
化学化工引用本文复制引用
刘博,韩晓东,张敏婷,徐方方,周文,焦红..AQC柱前衍生化高效液相色谱法测定宝乐果中的游离氨基酸[J].中山大学学报（自然科学版）,2013,52(4):100-104,5.基金项目
广东省科技现代生物技术资助项目(2011B010500014) （2011B010500014）
广州市科技天然活性物质开发利用研究资助项目(2011J4300077) （2011J4300077）
广东省科技项目农业攻关资助项目(2012A020602046) （2012A020602046）
