中山大学学报（自然科学版）2013，Vol.52Issue(4)：121-124,4.
贵州9个少数民族线粒体DNA Region V及Y染色体DYS287位点多态性研究
An Analysis on the Polymorphism of Region V of Mitochondrial DNA and Y-chromosome DYS287 Sites in 9 Ethnic Groups of Guizhou
摘要
Abstract
This study investigated the polymorphism of Region V of the mitochondrial DNA and Y-chromosome DYS287 sites in 9 ethnic groups of Guizhou to provide the referable molecular genetics data with related ethnic groups of Guizhou.Polymerase chain reaction-polyacrylamide SDS gel electrophoresis and Polymerase chain reaction-agarose gel electrophoresis were applied to detect the polymorphism of 9bp deletion of mitochondrial DNA noncoding region and Y-chromosome DYS287 sites.Two type of polymorphism in mitochondrial DNA were discovered:standard form and deletion form; the frequency of 9bp deletion of mtDNA in Gelao、She、Zhuang、Mulao、Maonan、Menggu、Hui、Qiang、Man people were:26.6％、25 ％、20％、18.3 ％、13.3 ％、10％、8.3 ％、6.7 ％、5 ％,respectively; two type of polymorphism in Y-chromosome DYS287:YAP + and YAP-,the frequency of YAP + in Gelao、Maonan were:20 ％ 、1.67 ％,respectively,others were 0.The data of polymorphism of Region V of mitochondrial DNA and Y-chromosome DYS287 sites were obtained in 9 ethnic groups of Guizhou and the genetic information were provided for ethnic groups.关键词
贵州/少数民族/线粒体DNA/DYS287/多态性Key words
Guizhou / ethnic groups / mitochondrial / DNA / polymorphism分类
生物学引用本文复制引用
任凌雁,何燕,张婷,王婵娟,官志忠,单可人..贵州9个少数民族线粒体DNA Region V及Y染色体DYS287位点多态性研究[J].中山大学学报（自然科学版）,2013,52(4):121-124,4.基金项目
贵州省科技计划资助项目(黔科合J [2011] 2119、黔科合SY [2010] 3001、黔科合SY [2012] 3135号) （黔科合J [2011] 2119、黔科合SY [2010] 3001、黔科合SY [2012] 3135号）
