作物学报2013，Vol.39Issue(7)：1148-1154,7.DOI:10.3724/SP.J.1006.2013.01148
水稻类病变突变体c5的遗传分析与目标基因的精细定位
Genetic Analysis and Gene Fine Mapping of Rice Lesion Mimic Mutant c5
摘要
Abstract
The lesion mimic mutant c5 was obtained by treating the seeds of japonica variety Zhonghua 11 with chemical mutagen EMS (ethyl methane sulfonate).The phenotypes and main agronomic traits of the c5 mutant were investigated.There were many nearly circular brown spots began to appear on c5 leaves at 3-leaf stage.These leaves were stained further with DAB and trypan blue respectively and indicated that there were excessive H2O2 production and clusters of dead cells in the lesion tissues.Compared with the wild-type,the height of c5 plant decreased from 110.4 cm to 74.6 cm,the number of effective tiller per plant and spikelet per panicle were reduced by 23.7％ and 28.5％ respectively,thousand-grain-weight and seed setting rate were significantly dropped.In addition,c5 is endowed a broad-spectrum resistance to bacterial blight resistance to ten Philippine races ofX.oryzae pv.oryzae (Xoo).Genetic analysis showed that the lesion mimic mutant c5 was controlled by a single recessive nuclear gene.Minghui 86 was crossed with c5 and 6269 individuals of F2 population and 18 pairs of molecular markers were used for gene mapping.And the gene was further mapping to rice chromosome 5 in a 102 kb region between STS markers S41 and S47.Sequence analysis of the 102 kb region revealed that there were 11 candidates for c5 gene.All the 11 candidate genes were different from those lesion mimic genes reported previously,which suggested that the phenotype of c5 may be controlled by a novel gene.关键词
水稻(Oryza sativa L.)/抗病/类病变/遗传分析/基因定位Key words
Rice (Oryza sativa L.)/ Resistance/ Lesion mimic/ Genetic analysis/ Gene mapping引用本文复制引用
陈红霖,向阳海,赵纪莹,尹德东,梁国华,翟文学,江光怀..水稻类病变突变体c5的遗传分析与目标基因的精细定位[J].作物学报,2013,39(7):1148-1154,7.基金项目
本研究由国家转基因生物新品种培育重大专项(2011ZX08009-001,003)和国家自然科学基金项目(31071379)资助. （2011ZX08009-001,003）
