作物学报2013，Vol.39Issue(7)：1223-1230,8.DOI:10.3724/SP.J.1006.2013.01223
水稻类病斑突变体spl31的遗传分析与基因定位
Genetic Analysis and Fine Mapping of a Lesion Mimic Mutant spl31 in Rice
摘要
Abstract
A lesion mimic mutant temporarily designated as spl31 was discovered in the progeny of an excellent indica restorer line Jinhui 10 with seeds treated by ethyl methane sulfonate (EMS).The mutant phenotype was normal before the three leaf period but with yellow bad spots on leaves after the four leaf period.Along with the growth and development of the plant,the number of spots didn't show significant difference,whereas the lesion area gradually enlarged and developed with tawny margin.As the spl31 matured,lesion spots interconnected between each other becoming a continuous piece,resulting in leaves' death.The observation by transmission electronic microscope (TEM) demonstrated that most grana lamellae in spl31 's chroloplasts were irregularly stacked.Histochemical analysis revealed that deep blue stained cells presented discrete dietilution,illustrating that the lesion mechanism of spl31 may be spontaneously generated.The photosynthetic data showed that the mutation of spl31 had no effect on PSII.Furthermore,both 1000-grain weight and seed setting rate of the mutant decreased as compared with the wild type.Genetic analysis suggested that spl31 was controlled by a single nuclear recessive gene.Nipponbare was crossed with the spl31 and 621 mutational F2 recessive plants were used for gene mapping.And finally,Spl31 locus was mapped on chromosome 12 near the centromere between ID104 and ID11 with physical distance of 383 kb and co-segregated with ID105.关键词
水稻(Oryza sativa L.)/类病斑突变/程序性细胞死亡/基因定位Key words
Oryza sativa L./ Lesion mimic mutants/ Programmed cell death/ Gene mapping引用本文复制引用
代高猛,朱小燕,李云峰,凌英华,赵芳明,杨正林,何光华..水稻类病斑突变体spl31的遗传分析与基因定位[J].作物学报,2013,39(7):1223-1230,8.基金项目
本研究由国家科技支撑计划项目(2011BAD35B02-05)和重庆市攻关项目(cstc2012ggC80002,cstc2010AA1019)资助. （2011BAD35B02-05）
