作物学报2013，Vol.39Issue(7)：1319-1324,6.DOI:10.3724/SP.J.1006.2013.01319
外源甜菜碱对干旱胁迫下小麦幼苗叶绿体抗氧化酶及psbA基因表达的调节
Effects of Betaine on Chloroplast Protective Enzymes and psbA Gene Expression in Wheat Seedlings under Drought Stress
摘要
Abstract
With the purpose of revealing the effect of exogenous glycine betaine (GB) on wheat seedlings,Aikang 58 was used as the experimental material,which has the characteristics of high yield and drought resistance.The leaves were sprayed with 0.1,1.0,and 10.0 mmol L-1 of GB during four-leaf stage,respectively,while the root was applied by 30％ polyethylene glycol (PEG-6000) to simulate the drought environment.The effects of exogenous GB treatment on the parameters of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD) activities,superoxide anion radicals (O2-) production rate,malondialdehyde (MDA),chlorophyll content,and relative water content (RWC) under drought stress were tested three days after treatment.Simultaneously,the relative transcriptional expression ofpsbA in chloroplast was determined by Real-time PCR assay.The results showed that drought stress obviously decreased the RWC and chlorophyll content of wheat leaves,reduced activities of SOD,CAT,and POD,increased MDA content and the production rate of reactive oxygen species,and suppressed psbA expression.Nevertheless,such stress reactions were alleviated by exogenous GB treatment,and this regulation effect showed a great correlation with GB concentration.These results indicated that exogenous GB could remove excess reactive oxygen,and retard the decrease of RWC and chlorophyll content by regulating antioxidant enzyme activities in wheat chloroplast under drought stress,enhance the transcriptional level ofpsbA gene,accelerate the turnover of D 1 protein in wheat chloroplast,and finally improve the drought resistance in wheat.关键词
小麦/甜菜碱/psbA基因/干旱胁迫/叶绿体抗氧化酶Key words
Triticum aestivum/ Betaine/ psbA gene/ Drought stress/ Chloroplast antioxidant enzyme引用本文复制引用
侯鹏飞,马俊青,赵鹏飞,张欢玲,赵会杰,刘华山,赵一丹,汪月霞..外源甜菜碱对干旱胁迫下小麦幼苗叶绿体抗氧化酶及psbA基因表达的调节[J].作物学报,2013,39(7):1319-1324,6.基金项目
本研究由国家自然科学基金项目(31000688)和河南省教育厅科学技术研究重点项目(13A210487)资助. （31000688）
