摘要
Abstract
Objective:To study the effect of Ulinastatin and Verapamil during ischemia-reperfusion injury of rat small intestine and assess the possible mechanism.Methods:Rat model of small intestine I/R injury was used. 50 SD rats were randomly divided into pseudo operation group(group A),ischemia-reperfusion group(group B),Ulinastatin group(group C),Verapamil group(group D) and Ulinastatin plus Verapamil group(group E). During the small intestine ischemia 45 minutes and reperfusion 2 hours,the level of TNF-α,IL-1βand MDA in the serum were determined;the pathological changes of intestinal mucosa were examined observed respectively.Results:There were evident pathological changes of the intestinal mucosa in group B;the levels of TNF-α,IL-1βand MDA in groups B,C,D,E were significantly higher than that in group A(P<0.05);the levels of TNF-α,IL-1βand MDA in groups C,D,E were significantly lower than that in group B(P<0.05).But there was no significant difference between B group and C group(P>0.05);the levels of TNF-α,IL-1βand MDA in groups C,D were obviously higher than that in group E(P<0.05).Conclusion:Ulinastatin and Verapamil can reduce rat intestinal ischemia-reperfusion injury. The protective effect will be more significant when they are combined.关键词
缺血再灌注/乌司他丁/维拉帕米/小肠Key words
Ischemia-reperfusion/Ulinastatin/Verapamil/Small intestine分类
医药卫生
