中药材2013，Vol.36Issue(4)：544-549,6.
甘肃铨水大黄的HPLC指纹图谱研究
Research on HPLC Fingerprint of Rheum palmatum from Gansu Province Called "Quan-shui-da-huang"
钮正睿 1刘洪宇 2张庆生 1曹进 1毛淑杰 3李先端3
作者信息
- 1. 中国食品药品检定研究院食品化妆品检定所,北京100050
- 2. 国家食品药品监督管理总局保健食品审评中心,北京100070
- 3. 中国中医科学院中药研究所,北京100700
- 折叠
摘要
Abstract
Objective:To establish an analysis method for HPLC fingerprint of Rheum palmatum collected from Gansu province (called "Quan-shui-da-huang"),and provide basis for quality control of Quan-shui-da-huang.Methods:The Cosmosil 5C18-PAQ column(250mm×4.6mm,5μm) was used with the mixture of 0.1％ acetic acid and acetonitrile as mobile phase in a gradient elution mode.The flow rate was 1mL/min,column temperature was 30℃ and detection wavelength was set at 280 nm.The similarity evaluation,principal component analysis(PCA) and cluster analysis of crude drugs collected from different habitats were carried out.Results:The analysis method of HPLC fingerprint was set up.The characteristic fingerprint was obtained by chemometrics methods.Conclusion:Under the selected chromatographic conditions,constituents in Rheum palmatum can be separated well.This method can be used for the quality control of Quan-shui-da-huang.关键词
铨水大黄/HPLC指纹图谱/相似度评价/主成分分析/聚类分析Key words
Quan-shui-da-huang (Rheum palmatum L.)/HPLC fingerprint/Similarity evaluation/Principal component analysis (PCA)/Cluster analysis分类
医药卫生引用本文复制引用
钮正睿,刘洪宇,张庆生,曹进,毛淑杰,李先端..甘肃铨水大黄的HPLC指纹图谱研究[J].中药材,2013,36(4):544-549,6.
