中药材2013，Vol.36Issue(4)：645-649,5.
基于响应曲面设计法筛选蚯蚓蛋白烧烫伤喷雾剂处方的研究
Optimization the Formulation of Eisemia foetida Protein Burn Spray by Response Surface Methodology
侯清华 1宋淑亮 2梁浩 1黄文欣 2吉爱国1
作者信息
- 1. 山东大学威海国际生物技术研发中心,山东威海264209
- 2. 山东大学(威海海洋学院,山东威海264209
- 折叠
摘要
Abstract
Objective:To optimize the formulation of Eisemia foetida protein (EFP)burn spray.Methods:A five-factor,three-level response surface method was employed ;The response variable was the proliferation effect of EFP on NIH3T3 cells.Results:The optimization formulation was as follows:the proportion of EFP,glycerol and mannitol was 0.91％,1.42％ and 5％,respectively; 0.02 mol/LNa2 HPO4 and 0.01mol/L citric acid buffer system corresponding pH value was 7.0.Conclution:The response surface method is reliable,efficient and suitable for optimizing the formulation of EFP burn spray.关键词
蚯蚓蛋白/喷雾剂/响应曲面法Key words
Eisemia foetida protein (EFP) / Spay / Response surface method分类
医药卫生引用本文复制引用
侯清华,宋淑亮,梁浩,黄文欣,吉爱国..基于响应曲面设计法筛选蚯蚓蛋白烧烫伤喷雾剂处方的研究[J].中药材,2013,36(4):645-649,5.
