中药材2013，Vol.36Issue(4)：650-653,4.
大孔吸附树脂对葛根中3种异黄酮类化合物的吸附分离研究
Adsorption and Isolation of Macroporous Resin for Three Isoflavonoids from Puerariae Lobatae Radix
摘要
Abstract
Objective:To study the adsorption function of macroporous resins for the separation and purification of three isoflavonoids (3 '-hydroxypuerarin,puerarin,daidzin) in Puerariae Lobatae Radix.Methods:The contents of 3'-hydroxypuerarin,puerarin,daidzin were determined by HPLC.D101,AB-8,XDA-5,HP-20 and other five resins were systematically investigated for their adsorption capability,adsorption isothermal curve,adsorption and elution properties with static and dynamic experiments.Results:Experimental results showed that macroporous resin XDA-5 owned optimum adsorption and elution parameters.Optimal conditions for adsorption and elution the three components were:per 1 mL solution contained Puerariae Lobatae Radix 0.5～0.8g,flow rate 2BV/h,adsorbed 3'-hydroxypuerarin,puerarin and daidzin could be desorbed by alcohol at flow rate 1BV/h.Conclusion:The purity of 3'-hydroxypuerarin,puerarin and daidzin of the product obtained from Puerariae Lobatae Radix with optimum technology is 23.39％,52.09％ and 19.21％,respectively.关键词
3’-羟基葛根素/葛根素/大豆苷/大孔吸附树脂/葛根/分离Key words
3 '-Hydroxypuerarin / Puerarin / Daidzin / Macroporous resin/ Radix puerariae lobatae / Separation分类
医药卫生引用本文复制引用
崔蕴慧,王治平,樊化,杨珂..大孔吸附树脂对葛根中3种异黄酮类化合物的吸附分离研究[J].中药材,2013,36(4):650-653,4.基金项目
广东省科技计划项目(2012A080204003) （2012A080204003）
