安徽农业科学2013,Vol.41Issue(13):5933-5935,5946,4.
4株降TSNAs细菌菌株的培养条件优化研究
A Preliminary Study on Four Bacteria Strains Fermentation Conditions for TSNA Decomposition
摘要
Abstract
[Objective] The study aimed to find out the optimal culture condition of four TSNA-degrading endophytic bacteria.[Method] Effects of different culture medium,culture time,culture temperature,culture pH on growth state of the bacteria were studied.The growth states of bacteria under different culture conditions were measured.The culture conditions of four bacteria have further been optimized.[Result] The results showed that:the optimal culture condition of WB3 was culture medium of LB,cultivation time of 30-42 h,temperature of 25-28 ℃,pH of 6.0.The optimal culture condition of TRM was culture medium of LB,cultivation time of 24-30 h,temperature of 25-28 ℃,pH of 8.0; The optimal culture condition of WB6 was culture medium of LB,cultivation time of 30-42 h,temperature of 28-31 ℃,pH of 9.0.The optimal culture condition of WB7 was culture medium of LB,cultivation time of 30-36 h,temperature of 31-34 ℃,pH of 8.0.[Conclusion] The study can provide scientific reference basis for further utilization of strains.关键词
降TSNA/内生细菌/培养条件Key words
TSNA-degrading/ Endophytic bacteria/ Culture condition分类
农业科技引用本文复制引用
肖晓,汪安云,李旭,雷丽萍,夏振远..4株降TSNAs细菌菌株的培养条件优化研究[J].安徽农业科学,2013,41(13):5933-5935,5946,4.基金项目
国家局重大专项项目(110201101035 JH-10). (110201101035 JH-10)