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4株降TSNAs细菌菌株的培养条件优化研究

肖晓 汪安云 李旭 雷丽萍 夏振远

安徽农业科学2013,Vol.41Issue(13):5933-5935,5946,4.
安徽农业科学2013,Vol.41Issue(13):5933-5935,5946,4.

4株降TSNAs细菌菌株的培养条件优化研究

A Preliminary Study on Four Bacteria Strains Fermentation Conditions for TSNA Decomposition

肖晓 1汪安云 2李旭 1雷丽萍 3夏振远1

作者信息

  • 1. 云南省烟草农业科学研究院,云南昆明650031
  • 2. 云南省烟草公司临沧市公司,云南临沧677099
  • 3. 云南农业大学农学与生物技术学院,云南昆明650201
  • 折叠

摘要

Abstract

[Objective] The study aimed to find out the optimal culture condition of four TSNA-degrading endophytic bacteria.[Method] Effects of different culture medium,culture time,culture temperature,culture pH on growth state of the bacteria were studied.The growth states of bacteria under different culture conditions were measured.The culture conditions of four bacteria have further been optimized.[Result] The results showed that:the optimal culture condition of WB3 was culture medium of LB,cultivation time of 30-42 h,temperature of 25-28 ℃,pH of 6.0.The optimal culture condition of TRM was culture medium of LB,cultivation time of 24-30 h,temperature of 25-28 ℃,pH of 8.0; The optimal culture condition of WB6 was culture medium of LB,cultivation time of 30-42 h,temperature of 28-31 ℃,pH of 9.0.The optimal culture condition of WB7 was culture medium of LB,cultivation time of 30-36 h,temperature of 31-34 ℃,pH of 8.0.[Conclusion] The study can provide scientific reference basis for further utilization of strains.

关键词

降TSNA/内生细菌/培养条件

Key words

TSNA-degrading/ Endophytic bacteria/ Culture condition

分类

农业科技

引用本文复制引用

肖晓,汪安云,李旭,雷丽萍,夏振远..4株降TSNAs细菌菌株的培养条件优化研究[J].安徽农业科学,2013,41(13):5933-5935,5946,4.

基金项目

国家局重大专项项目(110201101035 JH-10). (110201101035 JH-10)

安徽农业科学

0517-6611

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