安徽农业科学2013，Vol.41Issue(15)：6699-6701,3.
月季内生真菌分离及抗黑斑病活性菌株的筛选
Isolation and Screening Endophytic Fungi with Anti-black-spot Disease Activities
摘要
Abstract
[Objective] The aim was to isolate and screen the endophytic fungi with activity to blank spot disease.[Method] Various kinds of healthy Rosa chinensis varieties collected from Xi'an,Xianyang,Baoji and Weinan were as materials,and endophytic fungi were isolated from the stem and leaves of the R.chinensis and then purified through 2-3 times transferring.Endophytic fungi with activity to blank spot disease were screened by using improved punching method.[Result] Total 67 strains of endophytic fungi were obtained,and the samples collected from Xianyang had the highest colonization rate and isolation rate ; the colonization rate and isolation rate of endophytic fungi from the stem of R.chinensis were obviously higher than those from the leaf of R.chinensis.In the 67 strains,there were three strains to have activity to blank spot disease,and they were all from Baoji.[Conclusion] The research results lay the basis for further screening bio-control candidates of fungi and biological control of environment-friendly fungi.关键词
月季/内生真菌/黑斑病/分离/筛选Key words
Rosa chinensis / Endophytic fungi / Marssonina rosae / Isolation / Screening分类
农业科技引用本文复制引用
徐玲玲,陶贵荣,姚武伟,李英,王雄,郭斌..月季内生真菌分离及抗黑斑病活性菌株的筛选[J].安徽农业科学,2013,41(15):6699-6701,3.基金项目
国家自然科学基金项目(31000144) （31000144）
陕西省科技厅项目(2012JQ3017) （2012JQ3017）
陕西省西安市科技局项目[NC1206(3)] （3）
陕西省教育厅项目(12JK1106). （12JK1106）
