安徽农业科学2013，Vol.41Issue(15)：6742-6743,6745,3.
酮病对荷斯坦奶牛抗氧化系统和一氧化氮含量的影响
Effects of Ketosis on Anti-Oxidative System and NO Content of Holstein Dairy Cows
王银龙 1高树 1贺志锐 1刘强 1赛务加甫1
作者信息
- 1. 石河子大学动物科技学院,新疆石河子832000
- 折叠
摘要
Abstract
[Objective] To observe the change of biochemical indicators in serum of Xinjiang Holstein cow ketosis and disease pathogenesis.[Method] Xinjiang Holstein cows were selected as experimental animals,their serum were detected with ketone powder method and improved salicylaldehyde detection method.Nitrate reductase method was used for determination of determinationserum nitric oxide (NO) content and nitric oxide synthase (NOS) activity,DTNB chromogenic assay was used to determine GSH-Px activity,the xanthine oxidation method was used for the determination of T-SOD activity,thio Pakistanthan properly acid method was adopted for determination of serum MDA content.[Result] The results showed that ketosis cows serum GSH-Px activity poles (P ＜ 0.01) reduction in T-SOD activity significantly decreased (P ＜ 0.05) and serum MDA content significantly increased (P ＜ 0.05) ; NO content and NOS activity in the serum of cows in the control group were significantly greater than the positive group.[Conclusion] Ketosis not only affects the anti-oxidative system but also can reduce NO content and activity of NOS,which has important significance to reveal the mechanism of cow ketosis.关键词
荷斯坦奶牛/酮病/一氧化氮/抗氧化系统Key words
Holstein dairy cows / Ketosis / NO / Anti-oxidative system分类
农业科技引用本文复制引用
王银龙,高树,贺志锐,刘强,赛务加甫..酮病对荷斯坦奶牛抗氧化系统和一氧化氮含量的影响[J].安徽农业科学,2013,41(15):6742-6743,6745,3.
