北京师范大学学报（自然科学版）2013，Vol.49Issue(4)：369-373,5.
转基因烟草中过量生长素对叶片生长发育的影响
EFFECT OF OVER-PRODUCED IAA ON LEAF PHENOTYPE IN iaaM TRANSGENIC TOBACCO (NICOTIANA TABACUM L.)
摘要
Abstract
The correlation between endogenous IAA level and leaf phenotype was investigated by introducing tryptophan monooxidase gene (iaaM),or co-expression of iaaM and isopentenyl transferase (ipt) into tobacco (Nicotiana tabacum L.) plants via A.tumefaciens transfection.Semiquantitative RT-PCR,ELISA and microscopy were used,respectively,to analyze transgene expression level,to quantitate endogenous phytohormones (zeatin riboside-ZR,isopentenyl adenosine-IPA,indole-3-acetic acid-IAA),leaf stoma density,guard cell length,palisade tissue cell size and leaf cross-section.Data showed that in comparison with the wild type plants,transgenic plants-iaaM10 displayed the highest content in IAA but the lowest content in cytokinin; whereas iaaMipt3 showed the highest level in cytokinin.With increasing IAA levels,stomatal density decreased,whereas guard cell length,palisade tissue cell length and width,and thickness of leaf cross-section all increased.A possible effect of cytokinin-to-auxin ratio on plant phenotype was discussed.关键词
烟草/色氨酸单氧化酶基因/异戊烯基转移酶基因/生长素/细胞分裂素Key words
Nicotiana tabacum/ tryptophan monooxidase gene/ isopentenyl transferase gene/ auxin/cytokinin引用本文复制引用
李朝霞,殷娴,江一希,姜洋,肖尊安..转基因烟草中过量生长素对叶片生长发育的影响[J].北京师范大学学报（自然科学版）,2013,49(4):369-373,5.基金项目
国家转基因生物新品种培育科技重大专项基金资助项目(20092X08009-089B) （20092X08009-089B）
