北京生物医学工程2013，Vol.32Issue(4)：357-362,374,7.DOI:10.3969/j.issn.1002-3208.2013.04.06.
载药纳米磁性碘化油微乳用于肝肿瘤综合治疗的体外评价
Evaluation on the feasibility of the drug loaded magnetic nanocomposite iodized oil microemulsion on the comprehensive treatment for liver cancer:an in vitro investigation
摘要
Abstract
Objective Drug-loaded magnetic nanocomposite iodized oil microemulsion was prepared by using the double emulsion method.The microemulsion contained anti-tumor drug doxorubicin(DOX),magnetic nanoparticles(MNP),and iodized oil as the matrix.The evaluation on biocompatibility and the feasibility of the nanocomposite microemulsion as the media for liver cancer comprehensive treatment was carried out in vitro.Methods MNPs modified with oleic acid,DOX and iodized oil were made of composite microemulsion by phacoemulsification.Inductive heating properties were evaluated by monitoring the temperature increase of the nanocomposite microemulsion under an alternative magnetic field (AMF).Mouse fibroblast cell L-929 and human liver hepatocellular carcinoma cell HepG-2 were employed in the study.HepG-2 cells were subjected to various treatments including AMF exposure,drug treatment,heating treatment as well as thermochemotherapy treatment medicated by the nanocomposite microemulsion.Cell viability was evaluated by CCK-8 assay.Results The nanocomposite microemulsion possessed excellent inductive heating property under AMF exposure which satisfied the temperature requirement during cancer hyperthermia.Cytotoxicity study showed that the biocompatibility of the microemulsion was good,which met the requirement to Evalution on biology of medical apparatus GB/T 16886.Conclusions The biocompatibility of the microemulsion containing DOX and MNPs is good.The bi-modal treatment with magnetic thermochemotherapy is more effective than mono-modal treatment.The synergistic effect between heating treatment and drug treatment was confirmed under AMF exposure.The nanocomposite microemulsion is anew functional media for comprehensive treatment of liver tumors.关键词
载药纳米磁性碘化油微乳/磁感应热疗/肿瘤综合治疗/HepG-2Key words
drug-loaded magnetic nanocomposite iodized oil/ magnetic mediated hyperthermia/ cancer comprehensive treatment/ HepG-2分类
医药卫生引用本文复制引用
孔维超,张阳德,魏兰镔,庞瑞,谢乐,唐劲天,赵凌云..载药纳米磁性碘化油微乳用于肝肿瘤综合治疗的体外评价[J].北京生物医学工程,2013,32(4):357-362,374,7.基金项目
国家自然科学基金(81172182,81172120)资助 （81172182,81172120）
