重庆医学2013，Vol.42Issue(19)：2194-2197,4.DOI:10.3969/j.issn.1671-8348.2013.19.004
显微镜观察药敏检测技术检测结核分枝杆菌耐药性研究
Detection of drug susceptibility in mycobacterium tuberculosis by microscopic observation drug susceptibility assay
摘要
Abstract
Objective To construct the method of microscopic observation drug susceptibility(MODS) for assaying the drug susceptibility of mycobacterium tuberculosis(MTB),and evaluate its clinical value.Methods MODS assay was constructed by using 24-well cell culture plate.The test conditions were discussed.The drug susceptibility of 192 MTB clinical isolates to rifampin (RIF),isoniazid(INH),ethambatal(EMB),streptomycin(SM) and ofloxacin(OFX) was detected by MODS and the result was compared with absolute concentration method on Lowenstein-Jensen(LJ) medium.BACTEC-960 TB system was used to determine the drug susceptibility of MTB strains which obtained inconsistent results from MODS assay and LJ method.Results Results of MODS were obtained in a median time of 6 days.When using the absolute concentration method as standard,the sensitivity,specificity and accordance rate were 97.8％,98.0％ and 97.9％ in RIF,98.7％,94.8％ and 96.4％ in INH,88.4％,94.3％ and 92.1％in EMB,93.2 ％,99.0 ％ and 96.4 ％ in SM,96.4 ％,95.6 ％ and 95.8 ％ in OFX,respectively.Conclusion MODS assay can be used for rapid detecting of drug susceptibility in MTB.关键词
分枝杆菌/结核/抗药性/微生物敏感性试验/显微镜检查Key words
mycobacterium tuberculosis/ drug resistance/ microbial susceptibility tests / microscopy引用本文复制引用
黄自坤,熊国亮,姜碧霞,方乐,李玮婷,徐晓萌,李俊明..显微镜观察药敏检测技术检测结核分枝杆菌耐药性研究[J].重庆医学,2013,42(19):2194-2197,4.基金项目
江西省卫生厅科技计划资助项目(20081050) （20081050）
江西省科技支撑计划资助项目(2009BSB11219) （2009BSB11219）
江西省青年科学家培养计划资助项目(2009DQ01900). （2009DQ01900）
