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百色市献血人群中卡波氏肉瘤相关疱疹病毒DNA的套式PCR检测

陈源红 周正东 曾怡 唐华英 韦红玉 樊晓晖

重庆医学2013,Vol.42Issue(19):2204-2205,2.
重庆医学2013,Vol.42Issue(19):2204-2205,2.DOI:10.3969/j.issn.1671-8348.2013.19.007

百色市献血人群中卡波氏肉瘤相关疱疹病毒DNA的套式PCR检测

Detection of Kaposi's sarcoma associated hepersvirus DNA in baise blood-donor crowd by nested PCR

陈源红 1周正东 2曾怡 3唐华英 2韦红玉 2樊晓晖2

作者信息

  • 1. 广西医科大学微生物学教研室,南宁530021
  • 2. 右江民族医学院微生物学与免疫学教研室,广西百色533000
  • 3. 广西百色市中心血站 533000
  • 折叠

摘要

Abstract

Objective To understand the current epidemic situation of KSHV prevalence among blood donor crowd in Baise city.Methods There were 474 donors had been detected,including 259 male and 215 female.The KSHV DNA was detected by nested polymerase chain reaction(n-PCR).Then there was a 168 bp strip for positive results in the 1.5 % agarose gel electrophoresis.Results The DNA of KSHV was found in 9 of 474(1.90%).The infection rate of male was 1.93% (5/259) and that of female was 1.86% (4/215).The chi-square test analysis showed that the differences were not significant(P>0.05) between the two groups of male and female.Conclusion The DNA of KSHV in Base blood-donor crowd was found,there was no significant difference of infection rate in different gender of blood-donor.

关键词

献血人群/卡波氏肉瘤相关疱疹病毒/套式聚合酶链反应

Key words

blood-donor crowd/Kaposi's sarcoma associated hepersvirus/nested Polymerase chain reaction

引用本文复制引用

陈源红,周正东,曾怡,唐华英,韦红玉,樊晓晖..百色市献血人群中卡波氏肉瘤相关疱疹病毒DNA的套式PCR检测[J].重庆医学,2013,42(19):2204-2205,2.

基金项目

国家自然科学资金资助项目(81071345,81160535) (81071345,81160535)

广西壮族自治区教育厅桂教科研(2012 201204LX316). (2012 201204LX316)

重庆医学

OA北大核心CSCDCSTPCD

1671-8348

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