重庆医学2013,Vol.42Issue(19):2204-2205,2.DOI:10.3969/j.issn.1671-8348.2013.19.007
百色市献血人群中卡波氏肉瘤相关疱疹病毒DNA的套式PCR检测
Detection of Kaposi's sarcoma associated hepersvirus DNA in baise blood-donor crowd by nested PCR
摘要
Abstract
Objective To understand the current epidemic situation of KSHV prevalence among blood donor crowd in Baise city.Methods There were 474 donors had been detected,including 259 male and 215 female.The KSHV DNA was detected by nested polymerase chain reaction(n-PCR).Then there was a 168 bp strip for positive results in the 1.5 % agarose gel electrophoresis.Results The DNA of KSHV was found in 9 of 474(1.90%).The infection rate of male was 1.93% (5/259) and that of female was 1.86% (4/215).The chi-square test analysis showed that the differences were not significant(P>0.05) between the two groups of male and female.Conclusion The DNA of KSHV in Base blood-donor crowd was found,there was no significant difference of infection rate in different gender of blood-donor.关键词
献血人群/卡波氏肉瘤相关疱疹病毒/套式聚合酶链反应Key words
blood-donor crowd/Kaposi's sarcoma associated hepersvirus/nested Polymerase chain reaction引用本文复制引用
陈源红,周正东,曾怡,唐华英,韦红玉,樊晓晖..百色市献血人群中卡波氏肉瘤相关疱疹病毒DNA的套式PCR检测[J].重庆医学,2013,42(19):2204-2205,2.基金项目
国家自然科学资金资助项目(81071345,81160535) (81071345,81160535)
广西壮族自治区教育厅桂教科研(2012 201204LX316). (2012 201204LX316)