重庆医学2013，Vol.42Issue(20)：2315-2317,3.DOI:10.3969/j.issn.1671-8348.2013.20.002
maspin诱导不同分化的胃癌细胞凋亡及可能的作用机制
Apoptosis and related mechanism induced by maspin in gastric cancer cells
摘要
Abstract
Objective To investigate apoptosis and related mechanism induced by maspin in gastric carcinoma cell SGC-7901 and MKN-45.Methods pCDNA3.1-maspin vector was constructed and transfected into SGC-7901 and MKN-45 cells.Maspin gene were detected with RT-PCR and Western blotting,cell apoptosis was teseted by TUNEL,Bax and Bcl-2 are detected with Westernblotting.Results After transfected with pCDNA3.1-maspin,both RNA and protein of maspin are increased obviously whether in SGC-7901 or in MKN-45(P＜0.05).cell apoptosis is obvious.Bax is up-regulated,and Bcl-2 is down-regulated.Conclusion cells apoptosis induced by maspin may be related to up-regulate Bax and down-regulate Bcl-2.关键词
maspin/胃肿瘤/细胞凋亡Key words
words/maspin/stomach neoplasms/apoptosis引用本文复制引用
谯敏,牛司强,王丕龙..maspin诱导不同分化的胃癌细胞凋亡及可能的作用机制[J].重庆医学,2013,42(20):2315-2317,3.基金项目
重庆医科大学校办资助项目(XBYB2007021) （XBYB2007021）
重庆市卫生局资助项目(2008-2-121). （2008-2-121）
