环境工程学报2013，Vol.7Issue(8)：2904-2908,5.
复合式膜生物反应器中胞外聚合物提取方法综合评价
Assessment on extraction methods of EPS from hybrid membrane bioreactor
摘要
Abstract
Four methods,including high-speed centrifugation,ultrasonication,cation exchange resin,heating,were employed for extracting EPS of activated sludge from HMBR.Chemical analysis method was combined with excitation-emission matrix (EEM) and gel-permeating chromatography (GPC) to estimate the four methods.The experimental result revealed that heating method employing 60℃ for 30 minutes showed the best extracting effect and caused little damage to the cell.The EEM spectra showed that four methods all had good extract effect on fulvic-acid-like substances.As to humic-like and protein-like substances,heating was prior to other three methods.Gel-permeating chromatography showed that the molecule weight distribution of EPS extracted by four methods got little difference.The molecule weight (MW) of EPS distributed from 100 to 1 000 000,mainly concentrating between 100 and 10 000.关键词
膜生物反应器/胞外聚合物/提取方法/三维荧光光谱/凝胶渗透色谱Key words
HMBR / EPS / extracting method / EEM / GPC分类
资源环境引用本文复制引用
陈华,胡以松,王晓昌,金鹏康..复合式膜生物反应器中胞外聚合物提取方法综合评价[J].环境工程学报,2013,7(8):2904-2908,5.基金项目
国家自然科学基金重点项目(50838005) （50838005）
国家自然科学基金资助项目(51178376) （51178376）
