渔业科学进展2013，Vol.34Issue(4)：109-117,9.
条浒苔蛋白质的纤维素酶辅助提取及其性质
Cellulase-assisted extraction and properties of protein from Enteromorpha clathrata
摘要
Abstract
Using extraction ratio as the index,the extraction process of protein from Enteromorpha clathrata was investigated using single factor test and uniform design.Results showed that the optimal water extracting conditions were as follows.,pH 11.0,temperature 60℃,and extraction time 5h.The cellulase-assisted extraction condition were as follows:solid/ liquid ratio 1 ∶ 50,temperature 30℃,pH 5.5,hydrolysis duration 5.5h,and enzyme dose 5.0％.The sample was first treated with cellulose,then extracted by water under the above conditions,and the extraction rate was 30.22％～35.74％.In order to utilize the protein of E.clathrata,the properties of the extracted protein were also tested.The isoelectric point (pI) of the protein was 3; foaming capability peaked at pH 7 and was enhanced with the increase of the protein concentration; the highest foam stability at pH 3 and concentration of 2mg/ml; the highest emulsifying capability and emulsion stability at concentrations of 4 mg/ml and 2mg/ml,respectively.The oil adsorption ability of the protein was found to be 131.6％,and the maximum solubility was achieved at 60℃.关键词
条浒苔/蛋白质/纤维素酶/均匀设计/等电点/起泡性/乳化性Key words
Enteromorpha clathrata / Protein / Cellulase-assisted extraction/Uniform design / Isoelectric point / Foaming capacity/Emulsifying capability分类
化学化工引用本文复制引用
盘赛昆,姚东瑞,朱强,王淑军,王艳..条浒苔蛋白质的纤维素酶辅助提取及其性质[J].渔业科学进展,2013,34(4):109-117,9.基金项目
国家科技支撑计划项目(2012BAC07B00)和江苏省海洋生物技术重点实验室开放基金项目(2009HS07)共同资助 （2012BAC07B00）
