家畜生态学报2013，Vol.34Issue(8)：11-16,6.
山羊CREM基因的克隆与生物信息学分析
The Clone and Bioinformatics Analysis of CREM Gene in Goat (Capra hircus)
摘要
Abstract
This study aimed to clone the full length cDNA of the goat CREM gene.Total RNA was extracted from goat testis and the full length cDNA was obtained by the reverse transcription PCR and RACE method.The results showed that the full length of CREM cDNA was 1183 bp and with a 960 bp opening reading frame (ORF),which encoding 319 amino acids.The bioinformatics predictions showed that the CREM gene belongs to the bZIP transcription factor family,and signature motifs pKID 73-115 and BRLZ 259-316 were also found.The phylogenic tree based on CREM amino acid sequence among multiple species detected the closest relationship between goat and cattle.These results would be the theoretical foundation for the further study on the genetic control of CREM gene expression.关键词
山羊/CREM基因/RACE技术/克隆/生物信息学分析Key words
goat/CREM Gene/ RACE/ clone/ bioinformatic analysis分类
农业科技引用本文复制引用
施力光,荀文娟,张春香,岳文斌,侯冠彧,周汉林..山羊CREM基因的克隆与生物信息学分析[J].家畜生态学报,2013,34(8):11-16,6.基金项目
中央级公益性科研院所基本科研业务费专项资金(1630032012022) （1630032012022）
