家畜生态学报2013，Vol.34Issue(8)：54-60,7.
响应面法优化畜禽用凝结芽孢杆菌发酵培养基
Study on the Fermentation Medium of Bacillus coagulans Applied to Animal by Response Surface Method
摘要
Abstract
Researchs on fermentation medium of Bacillus coagulans strain applied to animal on singlefactor experiment basis have been done.Three important factors of starch,mix of soybean meal and fish meal(mass ratio was 2 to 1)and dipotassium hydrogen phosphate,which can influence the living bacteria amount,were screened through Plackett—Burman design and test.The results showed that the optimal medium was starch 10.6 g/L,mix of bean flour 13.5 g/L and fish meal 6.7 g/Land dipotassium hydrogen phosphate 2.72 g/L by using the steepest ascent path,center combination of design and response surface analysis.Under the optimized medium,the living bacteria amount of fermentation broth can reach 67 × 108CFU/mL.关键词
凝结芽孢杆菌/Plackett-Burman设计/响应面法/发酵培养基/优化Key words
Bacillus coagulans/Plackett-Burman design/ response surface method/ fermentation medium/ optimization分类
农业科技引用本文复制引用
高书锋,张德元,陈海荣,孙翔宇,周映华,胡新旭,周小玲,刘惠知..响应面法优化畜禽用凝结芽孢杆菌发酵培养基[J].家畜生态学报,2013,34(8):54-60,7.基金项目
长沙市科技攻关资金专项(K1003312-21) （K1003312-21）
湖南省科技厅重点项目(2011NK2013) （2011NK2013）
