寄生虫与医学昆虫学报2013，Vol.20Issue(2)：102-109,8.DOI:10.3969/j.issn.1005-0507.2013.02.006
全沟硬蜱Salp15蛋白家族成员基因的克隆与融合表达
CLONE AND EXPRESSION OF SALP15 SUPER FAMILY PROTEIN GENE FROM IXODES PERSULCATUS (ACARI: IXODIDAE)
摘要
Abstract
As one of main vectors for Lyme borreliosis,Ixodes persulcatus threats both innocent individuals and domestic livestock.Salp15,a 15-kDa salivary protein in Ixodid ticks,has been known to suppress against the activities of host immunity and enhance the transmission of Lyme borrelia.To determine Salp15-1ike gene in Ixodes persulcatus,the primers for RT-PCR against total RNA from nymph according to other Salp15 homologues were designed,and firstly the Salp15 protein entitling Ⅰ.p larva5 (HE820731) potentially interacting with the outer surface protein C (OspC) of Lyme spirochaeta was obtained.The deduced protein Ⅰ.p larva5 shows similarity to Salp15 from other speices,i.e.62.3％ (Ⅰ.scapularis in North America),74.5％ (Ⅰ.ricinus in Western Europe),59.4％ (Ⅰ.persulcatus in Siberia),67.6％ (Ⅰ.persulcatus in Japan) respectively.Ⅰ.p larva5 CDS was then subcloned into expression vector pMAL-c4X,expressing in E.coli Rosseta (DE3),induced by IPTG.The recombinant protein was confirmed expressed successfully by SDS-PAGE and western blot,and its relative molecular weights about 60 kDa with MBP Taq protein.This study lays the fundamental basis for the interacting between spirochaeta and Ixodes persulcatus and host reservoir.关键词
涎腺蛋白/全沟硬蜱/宿主免疫/克隆/表达Key words
Salp15 / Ixodes persulcatus/ host immunity/ clone/ expression引用本文复制引用
王欣,孙毅,牛思博,蒋宝贵..全沟硬蜱Salp15蛋白家族成员基因的克隆与融合表达[J].寄生虫与医学昆虫学报,2013,20(2):102-109,8.基金项目
国家自然科学基金资助项目(No.81271878 （）
No.30872196) （）
