实用临床医药杂志2013，Vol.17Issue(9)：9-12,4.DOI:10.7619/jcmp.201309003
促红细胞生成素对大鼠体外循环后肾脏保护作用的研究
Protective effect of erythropoietin on renal injury following cardiopulmonary bypass in rats
胡健 1殷仕宝 1叶阿奇 1钱云 1李德闽2
作者信息
- 1. 江苏省仪征市人民医院,江苏仪征,211400
- 2. 南京军区南京总医院,江苏南京,210002
- 折叠
摘要
Abstract
Objective To investigate the protective effect of pretreatment with erythropoietin (EPO) on renal injury following cardiopulmonary bypass (CPB),and to explore related mechanism.Methods Thirty male Sprague-Dawley rats were randomly divided into three groups:sham,CPB,and EPO (CPB + 3 000 U/kg EPO) groups.The flow-rate was maintained at ≥100 mL/(kg · min)for 1 h.Blood samples were collected at the beginning (T0) and the end of CPB (T1),and 0.5 (T2),1 h(T3),2 h (T4)and 24 h(T5) after CPB for assays of Cr and BUN.Renal samples were obtained 24 h after the operation for the determination of TNF-a,IL-1β,IL-6,MDA,CAT,SOD,and for histopathologic examination.Results Compared with those of the CPB group,the EPO group showed an obvious decrease in plasma of Cr and BUN except T0 (P ＜ 0.05).Levels of TNF-a,IL-1β,IL-6 and MDA in renal tissue of the EPO treated animals were also significantly lower than those of the CPB group (P ＜ 0.05).But the activities of SOD and CAT remained a marked increase in CPB group (P ＜ 0.05).Renal impairment with cast formation and tubular necrosis in the tubular epithelium was found in CPB group.These changes were markedly reversed in EPO group.Conclusions EPO could have potential effect on preventing CPB-induced renal injury,probably through its anti-in ammatory effect and antioxidant function.关键词
促红细胞生成素/体外循环/肾脏损伤/炎症反应/氧化应激Key words
erythropoietin/cardiopulmonary bypass/renal injury/inflammatory response/oxidative stress分类
医药卫生引用本文复制引用
胡健,殷仕宝,叶阿奇,钱云,李德闽..促红细胞生成素对大鼠体外循环后肾脏保护作用的研究[J].实用临床医药杂志,2013,17(9):9-12,4.
