实用临床医药杂志2013，Vol.17Issue(13)：1-3,7,4.DOI:10.7619/jcmp.201313001
黄芩茎叶总黄酮对过氧化氢诱导人脐静脉内皮细胞氧化损伤的保护作用及其机制
Study on the protective effect and mechanism of SSTF on human umbilical vascular endothelial cells oxidative injury induced by H2O2
曹致超 1周晓霞 1庞树华 2谭家成 1苏佩清1
作者信息
- 1. 扬州大学临床医学院,江苏扬州,225001
- 2. 扬州市中医院,江苏扬州,225002
- 折叠
摘要
Abstract
Objective To investigate the protective effects and mechanisms of SSTF on human umbilical vascular endothelial cells (HUVEC) following oxidative injury caused by hydrogen peroxide (H2O2).Methods On the basis of oxidative injury model of HUVEC,the cell vitality (OD value) was measured by MTT,MDA content,LDH activity,SOD activity of each group were measured by the method of biochemical assay,apoptosis ratio of each group was checked by FCM,and inhibition apoptosis protein Bcl-2 of each group were measured by the method of western-blot.Results When HUVEC were injured by H2O2,the cell vitality (OD value) and the activity of SOD declined,while the activity of LDH,the content of MDA,and the apoptosis ratio of HUVEC increased (P ＜ 0.05 or P ＜0.01).The SSTF (100-400 mg/L) significantly increased the cell vitality (OD value),inhibited activity of LDH,decreased the content of MDA,increased the activity of SOD,decreased the apoptosis rate of HUVEC injured by H2O2,and improved the level of Bcl-2 (P ＜ 0.05 or P ＜ 0.01).Conclusion The SSTF can ease the injury of HUVEC,and decrease the apoptosis of HUVEC injured by H2O2.The mechanism may concern that it can improve the level of Bcl-2 to inhibit the apoptosis of HUVEC.关键词
黄芩茎叶总黄酮/人脐静脉内皮细胞/过氧化氢/氧化损伤Key words
SSTF/ human umbilical vascular endothelial cells (HUVEC) / hydrogen peroxide (H2O2) / oxidative injury分类
医药卫生引用本文复制引用
曹致超,周晓霞,庞树华,谭家成,苏佩清..黄芩茎叶总黄酮对过氧化氢诱导人脐静脉内皮细胞氧化损伤的保护作用及其机制[J].实用临床医药杂志,2013,17(13):1-3,7,4.
