实用临床医药杂志2013，Vol.17Issue(15)：23-25,28,4.DOI:10.7619/jcmp.201315008
结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定
Separation of 5-fluorouracil, leucovorin calcium and oxaliplatin based on HPLC method
摘要
Abstract
Objective To investigate the detection of the amount of 5-fluorouracil,leucovorin calcium and oxaliplatin in the urine of colorectal cancer patients.Methods The urine of 4 colorectal cancer patients was used by HPLC to separate and analyse the three above mentioned drugs with flow rate (1 mL/min),mobile phase (phosphoric acid solution/methanol (80/20 v/v)),detection wavelength (270 nm) and column (Inertsil ODS (150 mm × 4.6 mm,5μm)).Results The detection limits of 5-fluorouracil,leucovorin calcium and oxaliplatin were 42.0,29.4,33.3 μg/L,respectively.The linear range was 1.0-1 000.0 mg/L.The relative standard deviation was 0.34 ％,0.98 ％,1.00 ％,and recovery rate was between 93.40 and 110.70 ％.Conclusion This method is simple,inertsil being fully separated,the result being reliable,which provides a base for future metabolomics research in the urine of patients with colorectal cancer.关键词
结直肠癌/尿液/高效液相色谱法/5-氟尿嘧啶/奥沙利铂/亚叶酸钙Key words
colorectal cancer/ urine/ HPLC/ 5-fluorouracil/ oxaliplatin/ leucovorin calcium分类
医药卫生引用本文复制引用
陈锐,阿依夏木,马玉花,丁远大,封顺..结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定[J].实用临床医药杂志,2013,17(15):23-25,28,4.基金项目
国家自然科学基金资助项目(21075105) （21075105）
新疆维吾尔自治区教育厅重点项目(XJEDU201010) （XJEDU201010）
