南京农业大学学报2013，Vol.36Issue(4)：111-115,5.DOI:10.7685/j.issn.1000-2030.2013.04.018
磷酸替米考星在肉鸡组织内残留消除规律研究
Elimination regularity and residues of tilmicosin phosphate in broiler tissues
摘要
Abstract
The elimination regularity and residues of tilmicosin phosphate in broiler tissues were detected at UV 280 nm by using improved high performance liquid chromatography(HPLC),with the proportion of acetonitrile,tetrahydrofuran,dibutylamine phosphate buffer and water was 10∶5∶5∶80 by the mobile phase.The results showed that the average recovery rates of tilmicosin were 77.02％-115.04％ and the detection limit of tilmicosin in muscle,liver and kidney were 0.010,0.025 and 0.025 μg· g-1,respectively.The quantitation limits in muscle,liver and kidney were 0.020,0.050 and 0.050 μg· g-1,respectively.After 7 days of oral administration at dosage of 16 mg·kg-1,the tilmicosin residues in liver,kidney and muscle tissues of broiler decreased significantly within 4 hours to 1 day after discontinuation.The highest level of tilmicosin residues occurred in liver and followed by kidney and the lowest in muscle tissues.According to the calculation by software WT 1.4,the withdrawal period of tilmicosin was 9.6,12.8 and 13.96 days in liver,kidney and muscle respectively,suggesting that the withdrawal period of tilmicosin phosphate for broiler using should be 14 days by the 95％ confidence interval analysis.关键词
磷酸替米考星/高效液相色谱/残留/肉鸡Key words
tilmicosin phosphate/ HPLC / residues / broiler分类
农业科技引用本文复制引用
杨迪,张春兰,刘彩,沈向荣,张晓辉,宗欣茹,王文超,鲍恩东..磷酸替米考星在肉鸡组织内残留消除规律研究[J].南京农业大学学报,2013,36(4):111-115,5.基金项目
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
