农业环境科学学报2013，Vol.32Issue(8)：1571-1576,6.DOI:10.11654/jaes.2013.08.012
多氯联苯污染土壤好氧降解菌群的分离与鉴定
Isolation and Identification of Aerobic Polychlorinated Biphenyls-Degrading Bacterial Strains in PCBs-contaminated Soils
摘要
Abstract
Growth of microorganisms was stimulated by incubating polychlorinated biphenyl-polluted soils in Wenling and Xiaoshan with biphenyl as sole carbon and energy source.After 4 weeks,a total of thirty five strains of bacteria which were able to grow on biphenyl-containing minimal medium plates were isolated.Sixty percent of them are gram-negative bacteria.The results of 16S rRNA gene sequencing showed that the dominant strains are Stenotrophomonas sp.and Pandoraea sp..We chose two well growing strains from each soil to further study PCB-degradation.The four strains are Corynebacterium sp.WLS,Bacillus sp.WL10,Microbacterium sp.XS4 and Citrobacter sp.XS7.All of them were cultured in fresh mineral medium containing 100 mg ·L-1 biphenyl and 2 mg· L-1 2,3,4,5-tetrachlorobiphenyl (PCB61) for five days.The residual concentrations of PCB61 determined by gas chromatography showed all the strains could degrade PCB61.Especially,the transformation rate of PCB61 by Corynebacterium sp.WL 8,isolated from Wenling soil,reached 50％.This result will benefit further research of genetic screening,cloning and restructuring,as well as providing guidance for the cultivation of PCBs-degrading bacteria.关键词
多氯联苯/好氧降解/分离与鉴定Key words
polychlorinated biphenyl/ aerobic degradation/ isolation and identification分类
资源环境引用本文复制引用
唐伟,李英,梁璐怡,朱优峰,王凤平..多氯联苯污染土壤好氧降解菌群的分离与鉴定[J].农业环境科学学报,2013,32(8):1571-1576,6.基金项目
国家自然基金委青年科学基金项目(20607019) （20607019）
浙江省教育厅项目(20070054) （20070054）
