农业生物技术学报2013，Vol.21Issue(8)：949-956,8.DOI:10.3969/j.issn.1674-7968.2013.08.008
毛白杨真核细胞翻译起始因子5A基因(PtoeIF5A4)的克隆与表达分析
Cloning and Expression Analysis of the Eukaryotic Translation Initiation Factor 5A Gene(PtoeIF5A4) in Populus tomentosa
摘要
Abstract
The eukaryotic translation initiation factor 5A (eIF5A) is the only cellular protein that contains the unique polyamine-derived amino acid,hypusine.Genetic and pharmacological evidences suggest that eIF5A is essential for cell survival and proliferation.However,the precise function of eIF5A is still unclear.In the present study,We isolated the full length PtoeIF5A4 (GenBank Accession number:HQ529482) CDS from white poplar based on bio-information analysis.Sequence analysis showed that the sequence of PtoeIF5A4 shared 99％ identity with the sequence of Populus trichocarpa.The completed ORF of PtoeIF5A4 was 483 bp and encoding 160 amino acid residues with a calculated molecular mass of 17.5 kD and a pI of 5.76.Real-time PCR results showed that PtoeIF5A4 expressed in various organs in poplar,especially in the stem.The expression of PtoeIF5A4 increased with growth time until one year.The expression of PtoeIF5A4 in 1 year P.tomentosa tree stem secondary xylem was 4 times than that of in 1 month.These results demonstrate that PtoeIF5A4 may play an important role in growth and development of trees secondary growth.关键词
毛白杨/PtoeIF5A4/次生生长Key words
Populus tomentosa/PtoeIF5A4/Secondary development引用本文复制引用
张利姣,张杰伟,陈亚娟,管阳,王宏芝,魏建华..毛白杨真核细胞翻译起始因子5A基因(PtoeIF5A4)的克隆与表达分析[J].农业生物技术学报,2013,21(8):949-956,8.基金项目
国家自然科学基金(No.30972392),国家高技术研究发展计划(863)项目(No.2011AA100201)和国家重点基础研究发展规划(973)项目(No.2012CB114501) （No.30972392）
