农药学学报2013，Vol.15Issue(4)：434-438,5.DOI:10.3969/j.issn.1008-7303.2013.04.11
解淀粉芽孢杆菌LJ1对实验鼠的急性毒性研究
Acute toxic effect of Bacillus amyloliquefaciens LJ1 on rodents
摘要
Abstract
Bacillus amyloliquefaciens LJ1 is a bacterium isolated from soil sample which showed good activity against melon powdery mildew.To identify the safety of LJ1 to animals and human beings,the acute toxicity of fermentation broth of LJ1 to rodents was tested,and the content of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP)as well as 6 hemogram indexes in treated mice were detected.The results showed that LJ1 has little toxicity on mice by stomach toxicity tests and rats by skin toxicity tests(LD50＞4640mg/kg);the content of AST,ALT and ALP in serum and the number of white blood cell,red blood cell and hemoglobin in blood of mice increased with significant difference(P＜0.05)comparing with that of the control group 3 days after treatment,all of the indexes recovered to normal level and there was no significant difference comparing with that of the control group(P＜0.05)14 days after treatment.The results suggested that the fermentation broth of LJ1 has some side effect on the viscera function of mice at preliminary stage,but that will be restored 14 days later.Therefore,it could be deduced that LJ1 is safe to animals and has potential in developing as biocontrol agent.关键词
解淀粉芽孢杆菌LJ1/生防菌/小鼠/急性毒性/安全性Key words
Bacillus amyloliquefaciens LJ1/biocontrol agent/mice/acute toxicity/safety分类
农业科技引用本文复制引用
李文明,谷医林,王远宏,李娟,徐明珠,梁红春..解淀粉芽孢杆菌LJ1对实验鼠的急性毒性研究[J].农药学学报,2013,15(4):434-438,5.基金项目
国家自然科学基金项目(31171892) （31171892）
天津星火科技项目(10ZHXHNC08300). （10ZHXHNC08300）
