青岛大学医学院学报2013，Vol.49Issue(3)：213-215,218,4.DOI:10.11712/qdyxy201303009
5-磷酸腺苷诱导低温对大鼠内毒素血症的影响
EFFECT OF HYPOTHERMIA INDUCED BY ADENOSINE 5'-MONOPHOSPHATE (5'-AMP) ON ENDOTOXEMIC RATS
摘要
Abstract
Objective To investigate the effect of hypothermia induced by adenosine 5'-monophosphate (5'-AMP) on endotoxemic rats.Methods Sixty male Wister rats were evenly randomized to four groups as control,lipopolysaccharide (LPS),5'-AMP-hypothermia therapy (HT),and 5'-AMP-hypothermia prevention (HP).An endotoxemia model was created by intraperitoneal injection of LPS in LPS group; For rats in HT group,5'-AMP was given for hypothermia intervention when the rat temper ature rose by 0.5 ℃ after intraperitoneal LPS; For HP group,a hypothermic animal model was created by injecting 5'-AMP followed by LPS for inflammation induction.ELISA method was applied to detect serum levels of inflammatory cytokine in rats at 3,6 and 12 hours after injection of LPS,their lung tissues were analyzed pathologically with hematoxylin-eosin staining.Results Compared with the rats in LPS group,the hypothermia induced by 5'-AMP could markedly decrease serum levels of TNF-α,IL-1β and IL-6 and increase IL-10 (F =2.50-26.73 ; q =6.36-214.85 ; P＜0.05).Pathologically,hypothermia induced by 5'-AMP obviously inhibited acute lung injury induced by LPS.Conclusion Hypothermia induced by 5'-AMP plays a noticeable role in the prevention and treatment of endotoxemia and acute pulmonary injury in rats caused by LPS.关键词
脂多糖类/内毒素血症/5-磷酸腺苷/低温/大鼠Key words
lipopolysaccharides/ endotoxemia/ adenosine 5'-monophosphate/ hypothermia/ rats分类
医药卫生引用本文复制引用
杜娜,卢恕来,潘新亭,苗志敏,江涛,王云龙..5-磷酸腺苷诱导低温对大鼠内毒素血症的影响[J].青岛大学医学院学报,2013,49(3):213-215,218,4.基金项目
青岛市科技局资助项目(11-2-4-2-(26)-jch （11-2-4-2-(26）
11-2-4-2-(24)-jch) （24）
