山西农业大学学报（自然科学版）2013，Vol.33Issue(3)：239-243,5.
猪、牛和兔粪中致病性大肠杆菌LEE毒力岛的检测分析
Detection and Analysis of Pathogenic Escherichia coli and LEE PAI in Porcine, Bovine and Rabbit Feces
摘要
Abstract
Many different kinds of germs were detected in animal feces which often act as important infectious sources when diseases occurred.The research was carried on to isolate Escherichia coli from the feces of the pig breeding farm,cattle farm and rabbit breeding farm,and then a PCR detection experiment of the eaeA gene on the LEE PAI was conducted to study the existence situation of the Escherichia coli owned LEE PAI in the animals.The experiment results showed that detection rate of Escherichia coli in pig,cattle and rabbit were 12.9 ％,14.0 ％,and 22.5 ％ respectively.The conclusions were that positive Escherichia colicarried with the eaeA gene was the main cause of diarrhea in animals.LEE pathogenicoty island could be a main method for EPEC molecular diagnosis and epidemiological investigation.关键词
大肠杆菌/LEE/毒力岛Key words
E.coli/LEE gene/PAI分类
农业科技引用本文复制引用
高荣琨,王丽芳,李锐,郑明学..猪、牛和兔粪中致病性大肠杆菌LEE毒力岛的检测分析[J].山西农业大学学报（自然科学版）,2013,33(3):239-243,5.基金项目
国家自然科学基金项目(31272536) （31272536）
山西省科技攻关项目(20120312008) （20120312008）
