天然产物研究与开发2013，Vol.25Issue(6)：799-801,3.
HPLC法测定不同产地鱼腥草中不同部位芦丁的含量
Determination of Rutin in Different Parts of Houttuynia cordata Thunb.Collected in Various Areas by HPLC
摘要
Abstract
The objective of this study is to establish a HPLC method for the determination of rutin in different parts of Houttuynia cordata Thunb..Eclipse XDB-C18(4.6 mm× 250 mm,5 μm) column was employed with 0.1％ phosphoric acid in water and methanol as mobile phases,the column temperature was set at 20 ℃.The flow rate was 1.0 mL/min and the detection wavelength was set at 358 nm.The linear range of rutin was 0.02 ～ 0.50 mg/mL,the correlation coefficient was 0.9999 and the average recoveries (n =3) was 92.00％.The results showed that the contents of rutin were different from various areas and different organs.The method is simple,rapid and reliable,it can be used to determine the content of rutin in traditional Chinese herbs.关键词
鱼腥草/芦丁/高效液相色谱法/含量测定Key words
Houttuynia cordata Thunb./ rutin/ HPLC / quantitative determination分类
医药卫生引用本文复制引用
李瑞玲,崔运启,刘奇森..HPLC法测定不同产地鱼腥草中不同部位芦丁的含量[J].天然产物研究与开发,2013,25(6):799-801,3.
